Job: Postdoctoral Research Scholar, North Carolina State University, Raleigh, NC

General Information

Job title:
Postdoctoral Research Scholar
Job location:
Raleigh, NC 27607 United States

Requisition code:
Date posted:
Job type:

Career path:

Scientific Discipline:

Job Description

The Department of Population Health and Pathobiology within the College of Veterinary Medicine is seeking applicants for a fellow to lead a project investigating the role of oral influenza vaccination in generation of lactogenic immune protection in swine and their suckling piglets. Duties would include planning and conducting experiments under BSL-2 bio-containment conditions; establishing procedures and techniques; writing and editing manuscripts/articles; and provide educational guidance to students. This position is part of a Gates Foundation-funded collaboration between Duke University and NCSU whose goal is to develop animal models of breast milk immunity after mucosal vaccination during the pre and postpartum periods. The project at NCSU aims to evaluate mucosal vaccines in the mother-neonatal dyad against influenza infection and to study the virus-host interactions in pig respiratory viral diseases. The ideal candidate would have a background in virology or immunology and have experience working with pigs.
The position requires the individual to display critical thinking and independent judgement as it relates to ongoing and new immunology, viral research with direct guidance from the Primary Investigator. The research is conducted at the BSL2 level of containment and utilizes the conventional pig animal model. The selected individual will participate in pig animal trials to evaluate the role of mucosal vaccination in generating lactogenic immunity against influenza infection including sample collection and processing. The candidate will establish and run immunological and virological assays to investigate the virus-host interaction and specific cellular responses to pig viral infections.
Duties & Responsibilities include:
• Conduct infectious disease research investigating influenza pathogenesis and vaccine studies as a member of a large, multi-disciplinary team.
• Experience in immunological techniques including immune cell isolation, flow cytometry, ELISA, EliSpot, immunoblotting, hemagglutionation assay and immunohistochemistry.
• Provide technical support including molecular biology techniques such as RT-PCR.
• Work with animals: pig sampling, bleeding, milking, isolation of mononuclear cells and serum/whey, nasal swabs, tissue processing at the BSL2 level of containment.
• Develop, plan, and record experiments.
• Write and edit manuscripts and/or articles for publications and/or presentations.
• Process and prepare experimental data for statistical analysis, interpret and evaluate data
• Training of new students/visiting scholars/collaborators.
• Provide oversight of daily programmatic activities.
• Provide lab support and ensure compliance with university regulations.
• It is anticipated that the selected individual will serve as a co-investigator and/or key personnel and contribute to preliminary data for intramural and extramural research grant proposals.

Job Requirements

• DVM or PhD
• RT-PCR, cell culture techniques, conducting safety and immunogenicity studies, immunological assays, virological assays
• Poster presentations and/or presentation at local, university or national meetings
• Ability to work with animals in a farm setting and in the BSL2 level of biocontainment
• Strong organizational skills and attention to detail
• Desire and willingness to learn, develop, and execute new experimental techniques and take on challenging scientific problems
• Ability to work independently as well as contribute to a supportive team environment.
• Ability to coordinate and prioritize multiple, concurrent projects.
• Knowledge of laboratory equipment and procedures.
• Accurate record keeping and data management skills.

• Preference for PhD in immunology, molecular biology, virology, or related field. or • For candidates that hold a DVM, preference is to have also earned an MS in related field.
• 5 years of laboratory experience
• Experience working or conducting studies with animals – previous experience with large animal models, preferentially pigs
• Molecular or virological laboratory experience – virus isolation, virus production/purification/titration
• Hands-on experience in primary cell isolation, cell culture techniques, conducting safety and immunogenicity studies in pigs,
• hands-on experience conducting ELISA, EliSpot assays in mammals or other immunological assays, flow cytometry, RT-PCR, hemaggutination assay
• Manuscript editing, poster presentations and/or presentation at local, university or national meetings, published articles in nationally/globally recognized scientific journals

(Job number: 4045368)